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assays zymopure ii plasmid midiprep kit zymo research d4200 experimental models  (Zymo Research)


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    Zymo Research assays zymopure ii plasmid midiprep kit zymo research d4200 experimental models
    Assays Zymopure Ii Plasmid Midiprep Kit Zymo Research D4200 Experimental Models, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+midiprep+kit/ZymoPURE+II+Plasmid+Midiprep+Kit/pm42905510-97-53-59
    Average 99 stars, based on 612 article reviews
    assays zymopure ii plasmid midiprep kit zymo research d4200 experimental models - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: CRISPR-Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella) patens.
    Article Snippet: .. DNA oligosnucleotide, containing protospacer sequence pMH-Cas9-sgRNA vector or pMK-Cas9-sgRNA (Bezanilla lab) PmeI restriction enzyme (New England Biolabs, cat. no. R0560) SnaBI restriction enzyme (New England Biolabs, cat. no. R0130) rCutSmart buffer, 10× (New England Biolabs, cat. no. B6004S) DNA gel recovery kit (Zymo Research, cat. no. D4001 or equivalent) TE buffer (Current Protocols, 2001) NEBuilder HiFi DNA assembly master mix (New England Biolabs, cat. no. E2621) Wu, Ryken and Bezanilla 3 of 19 Current Protocols DH5α competent E. coli cells LB medium plates with 100 μg/ml carbenicillin (see Current Protocols, 2001) LB medium (liquid) with 100 μg/ml carbenicillin (see Current Protocols, 2001) Plasmid miniprep kit (Zymo Research, cat. no. D4015 or equivalent) Plasmid midiprep kit (Zymo Research, cat. no. D4200 or equivalent) PCR thermocycler Gel electrophoresis equipment Spectrophotometer Incubator Incubator-shaker Design oligonucleotides containing protospacer sequence 1. ..

    Plasmid Preparation:

    Article Title: CRISPR-Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella) patens.
    Article Snippet: .. DNA oligosnucleotide, containing protospacer sequence pMH-Cas9-sgRNA vector or pMK-Cas9-sgRNA (Bezanilla lab) PmeI restriction enzyme (New England Biolabs, cat. no. R0560) SnaBI restriction enzyme (New England Biolabs, cat. no. R0130) rCutSmart buffer, 10× (New England Biolabs, cat. no. B6004S) DNA gel recovery kit (Zymo Research, cat. no. D4001 or equivalent) TE buffer (Current Protocols, 2001) NEBuilder HiFi DNA assembly master mix (New England Biolabs, cat. no. E2621) Wu, Ryken and Bezanilla 3 of 19 Current Protocols DH5α competent E. coli cells LB medium plates with 100 μg/ml carbenicillin (see Current Protocols, 2001) LB medium (liquid) with 100 μg/ml carbenicillin (see Current Protocols, 2001) Plasmid miniprep kit (Zymo Research, cat. no. D4015 or equivalent) Plasmid midiprep kit (Zymo Research, cat. no. D4200 or equivalent) PCR thermocycler Gel electrophoresis equipment Spectrophotometer Incubator Incubator-shaker Design oligonucleotides containing protospacer sequence 1. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), erythromycin (VWR, Cat# 0219), spectinomycin (Cat# BML‐A281‐0010), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..

    Polymerase Chain Reaction:

    Article Title: CRISPR-Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella) patens.
    Article Snippet: .. DNA oligosnucleotide, containing protospacer sequence pMH-Cas9-sgRNA vector or pMK-Cas9-sgRNA (Bezanilla lab) PmeI restriction enzyme (New England Biolabs, cat. no. R0560) SnaBI restriction enzyme (New England Biolabs, cat. no. R0130) rCutSmart buffer, 10× (New England Biolabs, cat. no. B6004S) DNA gel recovery kit (Zymo Research, cat. no. D4001 or equivalent) TE buffer (Current Protocols, 2001) NEBuilder HiFi DNA assembly master mix (New England Biolabs, cat. no. E2621) Wu, Ryken and Bezanilla 3 of 19 Current Protocols DH5α competent E. coli cells LB medium plates with 100 μg/ml carbenicillin (see Current Protocols, 2001) LB medium (liquid) with 100 μg/ml carbenicillin (see Current Protocols, 2001) Plasmid miniprep kit (Zymo Research, cat. no. D4015 or equivalent) Plasmid midiprep kit (Zymo Research, cat. no. D4200 or equivalent) PCR thermocycler Gel electrophoresis equipment Spectrophotometer Incubator Incubator-shaker Design oligonucleotides containing protospacer sequence 1. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), erythromycin (VWR, Cat# 0219), spectinomycin (Cat# BML‐A281‐0010), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..

    Nucleic Acid Electrophoresis:

    Article Title: CRISPR-Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella) patens.
    Article Snippet: .. DNA oligosnucleotide, containing protospacer sequence pMH-Cas9-sgRNA vector or pMK-Cas9-sgRNA (Bezanilla lab) PmeI restriction enzyme (New England Biolabs, cat. no. R0560) SnaBI restriction enzyme (New England Biolabs, cat. no. R0130) rCutSmart buffer, 10× (New England Biolabs, cat. no. B6004S) DNA gel recovery kit (Zymo Research, cat. no. D4001 or equivalent) TE buffer (Current Protocols, 2001) NEBuilder HiFi DNA assembly master mix (New England Biolabs, cat. no. E2621) Wu, Ryken and Bezanilla 3 of 19 Current Protocols DH5α competent E. coli cells LB medium plates with 100 μg/ml carbenicillin (see Current Protocols, 2001) LB medium (liquid) with 100 μg/ml carbenicillin (see Current Protocols, 2001) Plasmid miniprep kit (Zymo Research, cat. no. D4015 or equivalent) Plasmid midiprep kit (Zymo Research, cat. no. D4200 or equivalent) PCR thermocycler Gel electrophoresis equipment Spectrophotometer Incubator Incubator-shaker Design oligonucleotides containing protospacer sequence 1. ..

    Spectrophotometry:

    Article Title: CRISPR-Cas9 Genome Editing in the Moss Physcomitrium (Formerly Physcomitrella) patens.
    Article Snippet: .. DNA oligosnucleotide, containing protospacer sequence pMH-Cas9-sgRNA vector or pMK-Cas9-sgRNA (Bezanilla lab) PmeI restriction enzyme (New England Biolabs, cat. no. R0560) SnaBI restriction enzyme (New England Biolabs, cat. no. R0130) rCutSmart buffer, 10× (New England Biolabs, cat. no. B6004S) DNA gel recovery kit (Zymo Research, cat. no. D4001 or equivalent) TE buffer (Current Protocols, 2001) NEBuilder HiFi DNA assembly master mix (New England Biolabs, cat. no. E2621) Wu, Ryken and Bezanilla 3 of 19 Current Protocols DH5α competent E. coli cells LB medium plates with 100 μg/ml carbenicillin (see Current Protocols, 2001) LB medium (liquid) with 100 μg/ml carbenicillin (see Current Protocols, 2001) Plasmid miniprep kit (Zymo Research, cat. no. D4015 or equivalent) Plasmid midiprep kit (Zymo Research, cat. no. D4200 or equivalent) PCR thermocycler Gel electrophoresis equipment Spectrophotometer Incubator Incubator-shaker Design oligonucleotides containing protospacer sequence 1. ..

    Crocin Bleaching Assay:

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), erythromycin (VWR, Cat# 0219), spectinomycin (Cat# BML‐A281‐0010), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..

    Electroporation:

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), erythromycin (VWR, Cat# 0219), spectinomycin (Cat# BML‐A281‐0010), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..

    Cloning:

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), erythromycin (VWR, Cat# 0219), spectinomycin (Cat# BML‐A281‐0010), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), and ultralow temperature freezer. ..

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..

    other:

    Article Title: Microbiota-derived bile acids antagonize the host androgen receptor and drive anti-tumor immunity
    Article Snippet: 2-B-Mercaptoethanol , ThermoFisher , Cat # 21985023.

    Article Title: Variants of CAS12A nucleases and methods of making and use thereof
    Article Snippet: PCR amplifications were performed using the following parameters: 98° C. for 30 s; 25 cycles for PCR1 and 8 cycles for PCR2 (98° C. 10 s, 55° C. 20 s, 72° C. 30 s); 72° C. for 5 min; hold at 12° C. The PCR reactions were performed with Q5 High-Fidelity DNA Polymerase (New England BioLabs, Beverly, MA, United States).

    Article Title: SMAP design : a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation
    Article Snippet: The entry vectors were isolated (GeneJET Plasmid Miniprep kit, Thermo Fisher Scientific) and validated with Sanger sequencing (Mix2Seq, Eurofins Scientific).

    Saline:

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), Columbia Blood Agar (CBA) (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), Luria–Bertani (LB) broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), the vacuum‐pumping system, phosphate‐buffered saline (PBS) (Gibco, Cat# 10010‐031), centrifuge, polymerase chain reaction (PCR) amplifier, d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), carbenicillin (GoldBio, Cat# C‐103‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), 50 mL Tube Top Vacuum Filter System (0.22 mm) (Corning Life Sciences, Cat. #430320), and ultralow temperature freezer. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Genetic manipulations of nonmodel gut microbes
    Article Snippet: .. Primer star DNA polymerase (Takara, Cat# R045), Blue sapphire DNA polymerase (Takara, Cat# RR350), Plasmid Midiprep Kit (Zymo Research, Cat# D4201), DNA Clean and Concentrator (Zymo Research, Cat# D4003), Tryptic Soy Agar (BD, Cat# 236950), Brain Heart Infusion Agar (BD, Cat# 241830), CBA (BD, Cat# 279240), Horse blood (Hemostat Laboratories, Cat# 637291), LB broth (BD, Cat# BP1426), glycerol (Fisher Bioreagents, Cat# BP229), PBS (Gibco, Cat# 10010‐031), centrifuge, PCR amplifier, tetracycline (GoldBio, Cat# T‐101‐25), chloramphenicol (VWR, Cat# 0230), d ‐cycloserine (D) (TCI, Cat# C1189), gentamicin (G) (GoldBio, Cat# G‐400‐25), kanamycin (K) (GoldBio, Cat# K‐120‐25), thiamphenicol (Thiam) (Acros Organics, Cat# 455450250), anaerobic chamber, aerobic incubator, electroporation system, Thermo Scientific Nanodrop 2000, Gibson Assembly Cloning Kit (NEB, Cat# E5510S), Quick DNA fungal/bacterial kit (Zymo Research, Cat# D6005), ultralow temperature freezer, Direct‐zol RNA Microprep kit (Zymo Research, Cat# R2062), PrimeScript RT Reagent Kit (Takara, Cat# RR047A), real‐time quantitative PCR system (Applied Biosystems, ABI 7500). ..



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